splinker

Definitions

Abbreviation of sequencing primer linker.

abbreviation, alt-of

Examples

“1986, B. W. Kalisch, S. A. Krawetz, K. H. Schoenwaelder, J. H. van de Sande, Covalently linked sequencing primer linkers (splinkers) for sequence analysis of restriction fragments, Gene, 1986; 44(2-3): 263-70, doi: 10.1016/0378-1119(86)90190-3, PMID: 3023188, A new method for direct⟳ sequence analysis of DNA restriction fragments uses synthetic covalently linked complementary oligodeoxynucleotides, as universal sequencing primer linkers (splinkers). These splinkers were designed to contain⟳ an inverted repeat⟳ sequence which forms a double-stranded hairpin structure⟳ with a known restriction site. The splinkers were characterized by their ability to be self-ligating (dimerized) and by their restriction digest product analysis of both the monomer and dimer.”

“The process⟳ requires a total of 64 splinkers and 4096 anchors. Initially, a selected splinker is ligated to a selected anchor.”

CEFR level