Meaning of splinker | Babel Free

Definitions

Abbreviation of sequencing primer linker.

abbreviation, alt-of

Examples

“1986, B. W. Kalisch, S. A. Krawetz, K. H. Schoenwaelder, J. H. van de Sande, Covalently linked sequencing primer linkers (splinkers) for sequence analysis of restriction fragments, Gene, 1986; 44(2-3): 263-70, doi: 10.1016/0378-1119(86)90190-3, PMID: 3023188, A new method for direct sequence analysis of DNA restriction fragments uses synthetic covalently linked complementary oligodeoxynucleotides, as universal sequencing primer linkers (splinkers). These splinkers were designed to contain an inverted repeat sequence which forms a double-stranded hairpin structure with a known restriction site. The splinkers were characterized by their ability to be self-ligating (dimerized) and by their restriction digest product analysis of both the monomer and dimer.”

“The process requires a total of 64 splinkers and 4096 anchors. Initially, a selected splinker is ligated to a selected anchor.”

CEFR level