Meaning of pegRNA | Babel Free

Definitions

A specialized guide RNA used in prime editing, comprising a single guide RNA (sgRNA) extended with a primer binding site (PBS) and a reverse transcription template (RTT) that encodes the desired genetic edit.

countable, uncountable

Examples

“Prime editors (PEs), initially represented by PE1, employed a reverse transcriptase (RT) fused to an RNA-programmable nickase (Cas9n) and a prime editing guide⟳ RNA (pegRNA) to directly copy⟳ genetic information from the extension on the pegRNA into the target⟳ genomic locus.”

“Briefly, upon Cas9-mediated nicking of the target⟳ site, the extended tail of the pegRNA hybridizes with the free 3' end⟳, which serves as an RT template and encodes the desired edit to be incorporated into the genome.”

“Upon expression of this prime editing construct, a complex of PE and pegRNA scans the DNA for the target⟳ site.”

CEFR level