Meaning of pegRNA | Babel Free

Definitions

A specialized guide RNA used in prime editing, comprising a single guide RNA (sgRNA) extended with a primer binding site (PBS) and a reverse transcription template (RTT) that encodes the desired genetic edit.

countable, uncountable

Examples

“Prime editors (PEs), initially represented by PE1, employed a reverse transcriptase (RT) fused to an RNA-programmable nickase (Cas9n) and a prime editing guide RNA (pegRNA) to directly copy genetic information from the extension on the pegRNA into the target genomic locus.”

“Briefly, upon Cas9-mediated nicking of the target site, the extended tail of the pegRNA hybridizes with the free 3' end, which serves as an RT template and encodes the desired edit to be incorporated into the genome.”

“Upon expression of this prime editing construct, a complex of PE and pegRNA scans the DNA for the target site.”

CEFR level