Taq polymerase

Definitions

A thermostable DNA polymerase known for its relatively high rate of transcription errors.

Examples

“Another use⟳ of Taq polymerase is directly in DNA sequencing, where the high temperatures employed help⟳ reduce⟳ problems caused by secondary structure⟳ in the template and allow⟳ an increase⟳ in the stringency of primers used. Taq polymerase has, for the reasons mentioned earlier, become⟳ a very important tool for the molecular biologist.”

“Taq polymerase lacks a proofreading activity and as a result⟳ is unable to correct⟳ its errors.”

“If a PCR is performed with Taq polymerase and with 10,000 template molecules, producing a product that is 1000 bp long, 1% of the newly generated fragments will have⟳ a mutation in the first cycle.”

CEFR level